陈箐;程昉;宋晓峰;林佳伟;董洋;何炜;赵静;

• 1:大连知微生物科技有限公司

• 2:大连理工大学精细化工国家重点实验室

• 3:南京航空航天大学生物医学工程系

摘要(Abstract)：

分析辽河油田某区块不同来源油泥中微生物种类多样性、优势菌群和分布特征,探讨功能微生物在含油污染物的生物修复及提高原油采收率的应用可行性。采集辽河油田某区块3个油泥样品,利用第二代高通量测序技术测定油泥样品内微生物的16SrDNA V3-V4可变区序列,整理和统计各分类水平的微生物类群数,分析微生物的丰度、分布和多样性,以及不同位置油泥微生物多样性丰度的差异。结果表明:辽河油田某区块油泥中3组样品属水平的微生物类群数分别为51、135和213,不同来源样品中微生物种类及所占比例差异显著;优势菌群中含有多种已报道可作用原油的功能微生物,如芽孢杆菌属(Bacillus)和诺卡氏土壤菌属(Nocardiopsis)等;从油泥中筛选的菌株LH-03发酵液表面张力为29.3mN/m,对煤油和液体石蜡乳化层高度分别为6.1和5.7cm,经鉴定为枯草芽孢杆菌(Bacillus subtilis),与Pseudomonas aeruginosa CB-01复配使用,油泥降解实验除油率达到51.9%,石蜡降解率达到38.9%,填砂管驱油实验可在水驱基础上提高采收率12.7%。辽河油田油泥含有较丰富的微生物修复物质基础,具备通过生物刺激和强化技术实现原位生物修复的可行性。

关键词(KeyWords)：辽河油田;油泥;第二代测序技术;微生物多样性;功能菌种筛选

Abstract:

Keywords:

基金项目(Foundation):辽宁省“百千万人才工程”重点项目(2015-4);; 大连市科技计划项目(2015F11GH089)

作者(Author):陈箐;程昉;宋晓峰;林佳伟;董洋;何炜;赵静;

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