王静;林军章;宋欣;谭晓明;胡婧;陈健斌;

• 1:中石化胜利油田分公司石油工程技术研究院

• 2:山东省黄河三角洲可持续发展研究院管理中心

摘要(Abstract)：

从油田采出水中分离得到一株产脂肽的细菌,经鉴定为枯草芽孢杆菌,该菌株发酵液表面张力最低为28.00mN·m-1。分离提纯其代谢产物,经红外光谱分析表明为一种脂肽类生物表面活性剂。该脂肽类表面活性剂具有较低的临界胶束质量浓度(CMC),为3.3 mg·L-1,其水溶液最低表面张力为26.60 mN·m-1,油水界面张力为0.01mN·m-1。理化特性分析表明,该脂肽类生物表面活性剂具有耐温(120℃)、耐盐(2×105 mg·L-1),pH适应范围较广(6.0~10.0)等特性,对原油具有较强的乳化性能。物模驱油评价实验表明,在水驱基础上,可提高采收率9%以上。该研究为油田微生物采油工业化应用提供理论依据。

关键词(KeyWords)：生物表面活性剂;芽孢杆菌;脂肽;临界胶束质量浓度;油藏适应性;驱油性能

Abstract:

Keywords:

基金项目(Foundation):国家高技术研究发展计划(863计划)项目(2013AA064401)

作者(Author):王静;林军章;宋欣;谭晓明;胡婧;陈健斌;

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参考文献(References)：

• [1]李俊峰,刘丽.脂肽类生物表面活性剂的研究进展[J].化学与生物工程,2015,32(1):12-15.Li Junfeng,Liu Li.Research progress of lipopeptide biosurfactant[J].Chemistry&Bioengineering,2015,32(1):12-15.

• [2]吕应年,杨世忠,牟伯中.脂肽的分离纯化与结构研究[J].微生物学通报,2005,32(1):67-73.Lv Yingnian,Yang Shizhong,Mu Bozhong.Isolation and identification of a ipopeptide[J].Microbiology,2005,32(1):67-73.

• [3]朱海霞,方新湘,海日古丽.生物表面活性剂在油田开采中的应用[J].现代化工,2008,28(2):407-410.Zhu Haixia,Fang Xinxiang,Xeyrigul.Application of biosurfactants in petroleum exploitation[J].Modern Chemical Industy,2008,28(2):407-410.

• [4]李媛,陆晓滨,董贝磊,等.脂肽类生物表面活性剂生产的研究[J].食品工业与科技,2008,29(11):296-302.Li Yuan,Lu Xiaobin,Dong Beilei,et al.Reserch on production of lipopeptide biosurfactants[J].Science and Technology of Food Industry,2008,29(11):296-302.

• [5]Arima K,Kakinuma A,Tamura G.Surfactin acrystalline peptidelipid surfactant produced by bacillus subtilis:Isolation,characterization and its inhibition of fibrin clot formation[J].Biochem Biophys Res Commun,1968,31(3):488-496.

• [6]Javaheri M,Jenneman G,McInerney M,et al.Anaerobic production of a biosurfactant by bacillus licheniformis JF-2[J].Appl Environ Microbiol,1985,50(3):698-700.

• [7]张卉,郝国良,徐俊斌.生物表面活性剂产生菌的筛选及培养条件优化[J].沈阳师范大学学报:自然科学版,2011,29(2):293-296.Zhang Hui,Hao Guoliang,Xu Junbin.Screening of biosurfactant producing strain and optimization of its culture conditions[J].Journal of Shenyang Normal Univesity:Naturan Science Edition,2011,29(2):293-296.

• [8]Seghal K G,Anto T T,Joseph S B,et al.Optimization and characterization of a new lipopeptide biosurfactant produced by marine brevibacterium aureum MSA13in solid state culture[J].Bioresource Technology,2010,101(7):89-96.

• [9]Tomasz J,Marcin,Tomas R,et al.Isolation and characterization of two new lipopeptide iosurfactants produced by pseudomonas fluorescens BD5isolated from water from the Arctic Archipelago of Svalbard[J].Bioresource Technology,2010,101(15):18-23.

• [10]Stefan I,Wu Yongfu,Patrick S,et al.New surfactant classes for enhanced oil recovery and their tertiary oil recovery potential[J].Journal of Petroleum Science and Engineering,2010,71(1/2):23-29.

• [11]郝瑞霞,鲁安怀,王关玉.枯草芽孢杆菌对原油作用的初探[J].石油学报(石油加工),2002,18(5):15-20.Hao Ruixia,Lu Anhuai,Wang Guanyu.Metabolism of bacullus subtilis on Crude oils[J].Acta Petrolei Sinica(Petroleum processing Section),2002,18(5):15-20.

• [12]王大威,刘永建,杨振宇,等.脂肽生物表面活性剂在微生物采油中的应用[J].石油学报,2008,29(1):111-115.Wang Dawei,Liu Yongjian,Yang Zhenyu,et al.Application of surfactin in microbial enhanced oil recovery[J].Acta Petrolei Sinica,2008,29(1):111-115.

• [13]曹娟,刘怡辰,张振华,等.脂肽类生物表面活性剂产生菌的分离及特性研究[J].环境科学学报,2009,29(10):2056-2062.Cao Juan,Liu Yichen,Zhang Zhenhua,et al.Isolation and characterization of lipopeptide tabiosufactant-producing microoranism[J].Acta Scientiae Circumstantiae,2009,29(10):2056-2062.

• [14]余焱,张志坚,宋柏函.一株高效生物表面活性剂产生菌的分离、鉴定及培养条件的优化[J].复旦大学学报:自然科学版,2012,51(4):501-506.Yu Yan,Zhang Zhijian,Song Baihan.solation,Identification and culture conditions optimization of a high-efficiency bio-surfactant producing bacterium[J].Journal of Fudan University:Natural Science Edition,2012,51(4):501-506.

• [15]罗建波,吴卫霞,张凡,等.一株产脂肽类生物表面活性剂菌株的分离及代谢产物分析[J].化学与生物工程,2010,27(2):46-49.Luo Jianbo,Wu Weixia,Zhang Fan,et al.Solation and metabolite analysis of a strain producing lipopeptide biosurfactant[J].Chemisstry&Bioengineerring,2010,27(2):46-49.

• [16]李宝庆,鹿秀云,郭庆港,等.枯草芽孢杆菌BAB-1产脂肽类及挥发性物质的分离和鉴定[J].中国农业科学,2010,43(17):3547-3554.Li Baoqing,Lu Xiuyun,Guo Qinggang,et al.Isolation and identification of lipopeptides and volatile compounds produced by bacillus subtilis strain BAB-1[J].Scientia Agricultura Sinica,2010,43(17):3547-3554.