任国领;曲丽娜;乐建君;由春梅;黄永红;

• 1:大庆师范学院生命科学学院

• 2:大庆油田有限责任公司勘探开发研究院

摘要(Abstract)：

应用分子生态学方法,从大庆油田萨南开发区高台子油层样品(包括1个注入水样和8口油井产出液样组成的混合样)中提取总DNA,扩增其16S rDNA片段,克隆到大肠杆菌,建立16S rDNA克隆文库,研究细菌群落结构.结果表明:从注入水样的微生物得出22个分类操作单元(OUT),从油井产出液样的微生物得出9个分类操作单元(OUT),注入水样的微生物多样性明显高于油井产出液样的.对克隆测序的16S rDNA序列进行GenBank数据库比对和系统发育分析,注入水样的细菌属于γ变形菌纲(79.0%)、β变形菌纲(3.0%)和未培养微生物(18.0%);油井产出液样的细菌属于γ变形菌纲(87.7%)、δ变形菌纲(8.5%)和未培养微生物(15.1%).与石油烃降解和产生物表面活性剂相关的假单胞菌(Pseudomonas)在注入水文库和油井产出液文库中的比例分别为51.0%和87.7%.油藏微生物群落结构的研究可以为微生物采油技术提供基础资料.

关键词(KeyWords)：高台子油层;16S rDNA克隆文库;细菌群落结构;注入水;产出液;微生物采油

Abstract:

Keywords:

基金项目(Foundation):大庆油田有限责任公司科技项目(dqc-2009-sc-ky001);;

黑龙江省教育厅科学技术研究项目(11553005)

作者(Author):任国领;曲丽娜;乐建君;由春梅;黄永红;

Email:

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