任国领;李蔚;施寒清;卞立红;戚桂娜;胡敏;

• 1:黑龙江省油田应用化学与技术重点实验室

• 2:大庆师范学院生物工程学院

• 3:大庆油田有限责任公司勘探开发研究院

• 4:东北石油大学石油工程学院

摘要(Abstract)：

从大庆油田含聚污水中分离得到3株聚丙烯酰胺降解菌(N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1),进行生理生化检测和16SrDNA分子生物学鉴定,在不同pH条件下混合培养,研究3株聚丙烯酰胺降解菌的最优降解组合、降解pH和降解时间。结果表明:3株聚丙烯酰胺降解菌分别属于纤维微菌属、不动杆菌属和假单胞菌属。在pH为7.0时,N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1组合的降解率最大,最优降解时间为7d时,降解率为39.5%;在pH为9.0时,N2-2-P139-D1和N2-2-P140-X1组合的降解率最大,最优降解时间为5d时,降解率为34.5%。复合菌N2-2-P139-D1和N2-2-P140-X1,以及复合菌N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1对现场含聚污水中聚丙烯酰胺的最大降解率分别为35.4%和31.0%,最优降解时间为5d。该结果为微生物在大庆油田采出液油水分离和含聚污水处理提供支持。

关键词(KeyWords)：聚丙烯酰胺;复合菌;降解率;含聚污水;大庆油田

Abstract:

Keywords:

基金项目(Foundation):黑龙江省青年创新人才计划项目(UNPYSCT-2015116);; 大庆市科技局项目(zd-2017-43)

作者(Author):任国领;李蔚;施寒清;卞立红;戚桂娜;胡敏;

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