任国领;袁红梅;侯兆伟;卞立红;黄永红;

• 1:大庆师范学院生命科学学院

• 2:大庆油田有限责任公司勘探开发研究院

摘要(Abstract)：

16S rDNA基因克隆文库的建立和DGGE(变性梯度凝胶电泳)是分析油藏微生物群落结构和种群动态变化的重要方法,油藏微生物基因组DNA的提取是进行16S rDNA基因克隆文库的建立和DGGE的前提.建立大庆油田油藏微生物基因组DNA提取的方法,与基于SDS细胞裂解法相比,华舜基因组抽提试剂盒法具有DNA浓度高、纯度好和省时等优点.不同油藏条件下的实验和PCR扩增技术分析结果表明:该方法能够成功提取基因组DNA,并且提取的基因组DNA可以直接用于克隆文库和DGGE实验的细菌16S rDNA PCR扩增.这说明DGGE与分子克隆相结合的分子生物学方法在研究微生物提高原油采收率(MEOR)机理及指导MEOR在油田生产中的应用有着重要意义.

关键词(KeyWords)：油藏微生物;基因组DNA;16S rDNA;PCR扩增

Abstract:

Keywords:

基金项目(Foundation):黑龙江省科技攻关项目(GZ05A406);;

大庆油田有限责任公司科技项目(dqc-2009-cc-ky001)

作者(Author):任国领;袁红梅;侯兆伟;卞立红;黄永红;

Email:

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