柏璐璐;

• 1:大庆油田有限责任公司勘探开发研究院

摘要(Abstract)：

为提高内源微生物驱采收率,调查大庆油田聚驱后区块的油藏微生物,并采集油水样,分析内源微生物的总数、类型和分布规律.研究聚驱后油藏中的细菌和古菌,利用PCR-DGGE指纹图谱、16SrDNA克隆文库的序列的生物进化树(确立微生物种类)和油藏微生物群落的16SrDNA多态性,得到内源微生物群落的16SrRNA基因分布图谱,从分子水平上分析油藏不同区块中内源细菌和古菌的分布情况、群落结构、生物多样性,以及微生物群落中各种细菌和古菌的组成及其种群大小.结果表明:聚驱后油藏油井产出水中内源微生物数量为103～104个/mL,大庆聚驱后油藏中存在丰富多样的微生物资源,非常适合内源微生物采油技术.这种驱油模式为聚驱后区块内源微生物激活剂的选择,以及内源微生物驱油机理提供参考.

关键词(KeyWords)：分子生物学;大庆油田;内源微生物;聚合物驱;基因文库;多样性;资源评价

Abstract:

Keywords:

基金项目(Foundation):中国石油科技攻关项目(2009AA063504)

作者(Author):柏璐璐;

Email:

参考文献(References)：

• [1]王大威,杨振宇,石梅,等.分子生物学在石油微生物多样性研究中的应用[J].生物技术,2005,15(5):86-89.

• [2]王凤兰,王晓东.油藏微生物群落分布及提高采收率的模拟实验研究[J].应用与环境生物学报,2007,13(6):859-862.

• [3]Gerrit V,Stephen M A,Monika F R,et al.Charac terization of 16SrRNA genes from oilfield microbial communities indicates thepresence of a variety of sulfate-reducing,fermentative,and sulfide-oxidizing bacteria[J].Appl.Envir.Microbiol,1996,62(5):1623-1629.

• [4]Yoh T,Miyuki N,Toshihiro H,et al.Distribution and physiological characteristics of hyperthermophiles in the Kubiki oil reservoir inNiigata,Japan[J].Appl.Envir.Microbiol,2000,66(1):73-79.

• [5]Sharon A C,Jennifer P,Perry F,et al.Isolation and characterization of a mycobacterium species capable of degrading three and four-ring aromatic and aliphatic hydrocarbons[J].Appl.Envir.Microbiol,1999,65(2):549-552.

• [6]Kazuya W,Kanako W,Yumiko K,et al.Molecular characterization of bacterial populations in petroleum-contaminated groundwaterdischarged from underground crude oil storage cavities[J].Appl.Envir.Microbiol,2000,66(11):4803-4809.

• [7]Yumiko K,Kazuya W.Isolation and characterization of a sulfur-oxidizing chemolithotroph growing on crude oil under anaerobic condi-tions[J].Appl.Envir.Microbiol,2003,69(1):107-112.

• [8]Elizaveta A B,Margarita L M,Alexander V L,et al.Radioisotopic,culture-based,and oligonucleotide microchip analyses of ther-mophilic microbial communities in a continental hightemperature petroleum reservoir[J].Appl.Envir.Microbiol,2003,69(10):6143-6151.

• [9]Kazuya W,Yumiko K,Natsuko H,et al.Diversity,abundance,and activityofarchaeal populations in oil-contaminated groundwateraccumulated atthe bottom of an underground crude oil storage cavity[J].Appl.Envir.Microbiol,2002,68(8):3899-3907.